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Miralles de Imperial-Ollero, Juan A.; Gallego-Ortega, Alejandro; Norte-Muñoz, María; Di Pierdomenico, Johnny; Bernal-Garro, José Manuel; Valiente-Soriano, Francisco J.; Vidal-Sanz, Manuel

Short- and Long-Term Study of the Impact of Focal Blue Light-Emitting Diode-Induced Phototoxicity in Adult Albino Rats

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  • Medientyp: E-Artikel
  • Titel: Short- and Long-Term Study of the Impact of Focal Blue Light-Emitting Diode-Induced Phototoxicity in Adult Albino Rats
  • Beteiligte: Miralles de Imperial-Ollero, Juan A.; Gallego-Ortega, Alejandro; Norte-Muñoz, María; Di Pierdomenico, Johnny; Bernal-Garro, José Manuel; Valiente-Soriano, Francisco J.; Vidal-Sanz, Manuel
  • Erschienen: MDPI AG, 2021
  • Erschienen in: International Journal of Molecular Sciences
  • Sprache: Englisch
  • DOI: 10.3390/ijms22189742
  • ISSN: 1422-0067
  • Schlagwörter: Inorganic Chemistry ; Organic Chemistry ; Physical and Theoretical Chemistry ; Computer Science Applications ; Spectroscopy ; Molecular Biology ; General Medicine ; Catalysis
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Background: In adult rats we study the short- and long-term effects of focal blue light-emitting diode (LED)-induced phototoxicity (LIP) on retinal thickness and Iba-1+ activation. Methods: The left eyes of previously dark-adapted Sprague Dawley (SD) rats were photoexposed to a blue LED (20 s, 200 lux). In vivo longitudinal monitoring of retinal thickness, fundus images, and optical retinal sections was performed from 1 to 30 days (d) after LIP with SD-OCT. Ex vivo, we analysed the population of S-cone and Iba-1+ cells within a predetermined fixed-size circular area (PCA) centred on the lesion. Results: LIP resulted in a circular focal lesion readily identifiable in vivo by fundus examination, which showed within the PCAs a progressive thinning of the outer retinal layer, and a diminution of the S-cone population to 19% by 30 d. In parallel to S-cone loss, activated Iba-1+ cells delineated the lesioned area and acquired an ameboid morphology with peak expression at 3 d after LIP. Iba-1+ cells adopted a more relaxed-branched morphology at 7 d and by 14–30 d their morphology was fully branched. Conclusion: LIP caused a progressive reduction of the outer retina with loss of S cones and a parallel dynamic activation of microglial cells in the lesioned area.</jats:p>
  • Zugangsstatus: Freier Zugang